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C7512

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Butyrylcholinesterase from equine serum

lyophilized powder, ≥10 units/mg protein

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Synonym(s):
Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥10 units/mg protein

mol wt

tetramer 440 kDa

composition

Protein, ≥60%

solubility

cold water: 60 U/mL

storage temp.

−20°C

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This Item
C1057C4290SRE0020
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

cold water: 60 U/mL

solubility

H2O: soluble 10 mg/mL

solubility

-

solubility

-

mol wt

tetramer 440 kDa

mol wt

tetramer 440 kDa

mol wt

-

mol wt

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

400, 600

General description

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). It is also termed as pseudocholinesterase. It is produced in the liver. It is found in blood, the synapse of neuromuscular junctions and glia cells and axons of white matter.

Application

Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine.

Biochem/physiol Actions

Butyrylcholinesterase (BChE) can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is activated by Ca2+ and Mg2+ and the activity is constant over the pH range 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamates.
Mutations in butyrylcholinesterase (BChE) gene results in the failure of respiratory system, prolonged paralysis and apnea.

Unit Definition

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Physical form

Lyophilized powder containing buffer salts

Preparation Note

This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder.

Analysis Note

Protein determined by biuret

inhibitor

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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