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  • Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.

Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.

PloS one (2014-01-07)
José Dijair Antonino de Souza Júnior, Roberta Ramos Coelho, Isabela Tristan Lourenço, Rodrigo da Rocha Fragoso, Antonio Américo Barbosa Viana, Leonardo Lima Pepino de Macedo, Maria Cristina Mattar da Silva, Regina Maria Gomes Carneiro, Gilbert Engler, Janice de Almeida-Engler, Maria Fatima Grossi-de-Sa
ABSTRACT

The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita proteases could play important roles in nematode parasitism, besides their function in ordinary digestion of giant cell contents for feeding. The precise roles of these proteins during parasitism however are still unknown, making them interesting targets for gene silencing to address protein function. In this study we have knocked-down an aspartic (Mi-asp-1), a serine (Mi-ser-1) and a cysteine protease (Mi-cpl-1) by RNAi interference to get an insight into the function of these enzymes during a host/nematode interaction. Tobacco lines expressing dsRNA for Mi-ser-1 (dsSER), Mi-cpl-1 (dsCPL) and for the three genes together (dsFusion) were generated. Histological analysis of galls did not show clear differences in giant cell morphology. Interestingly, nematodes that infected plants expressing dsRNA for proteases produced a reduced number of eggs. In addition, nematode progeny matured in dsSER plants had reduced success in egg hatching, while progeny resulting from dsCPL and dsFusion plants were less successful to infect wild-type host plants. Quantitative PCR analysis confirmed a reduction in transcripts for Mi-cpl-1 and Mi-ser-1 proteases. Our results indicate that these proteases are possibly involved in different processes throughout nematode development, like nutrition, reproduction and embryogenesis. A better understanding of nematode proteases and their possible role during a plant-nematode interaction might help to develop new tools for phytonematode control.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Extract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
Sigma-Aldrich
Extract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications