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XNAP2

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Extract-N-Amp™ Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

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Synonym(s):
Plant direct PCR kit
NACRES:
NA.55

usage

sufficient for 100 amplifications
 sufficient for 100 extractions
 sufficient for 100 reactions

Quality Level

200

feature

dNTPs included
hotstart

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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This Item
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Extract-N-Amp™ Plant PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

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Extract-N-Amp™ Plant PCR Kit

Extract-N-Amp™ Seed PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

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REDExtract-N-Amp™ Plant PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

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XNAP

REDExtract-N-Amp™ Plant PCR Kit

Extract-N-Amp™ Tissue PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

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XNAT2

Extract-N-Amp™ Tissue PCR Kit

usage

sufficient for 100 amplifications

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

colorless

color

-

color

red

color

colorless

XNAP2E

-

XNAPE, XNAPR, XNAPS

XNAT2R

application(s)

agriculture

application(s)

agriculture

application(s)

-

application(s)

-

General description

The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.

Application

Extract-N-Amp™ Plant PCR Kit has been used for genomic DNA isolation from plants and the fungal mycelium. It has also been used in polymerase chain reaction (PCR).
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Features and Benefits

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Components

Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

XNAP

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPE

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications

XNAR

Extract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPR

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E8155

Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

D5688

Dilution Solution, Extract-N-Amp Plant Dilution Solution

XNAPG

SYBR® Green Extract-N-Amp™ Plant PCR Kit, sufficient for 100 preparations

S4320

SYBR® Green Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Elin Videvall et al.
mSystems, 2(6) (2017-11-29)
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and
Ante Karoglan et al.
Acta dermato-venereologica, 99(13), 1253-1257 (2019-10-02)
Imbalance in skin microflora, particularly related to certain Cutibacterium acnes strains, may trigger acne. Application of non-acne-causing strains to the skin may modulate the skin microbiome and thereby lead to a reduction in acne. This pilot study evaluates the safety
Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas
Obase K, et al.
Mycoscience (2021)
Jessica M Vélez et al.
PloS one, 16(1), e0231367-e0231367 (2021-01-07)
The ectomycorrhizal fungal symbiont Cenococcum geophilum is of high interest as it is globally distributed, associates with many plant species, and has resistance to multiple environmental stressors. C. geophilum is only known from asexual states but is often considered a
Len N Gillman et al.
Journal of evolutionary biology, 23(6), 1327-1330 (2010-05-12)
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer
View All Related Papers

Articles

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service