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E0781

Sigma-Aldrich

Extract-N-Amp PCR ReadyMix with qPCR Reference dye

125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

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NACRES:
NA.55

description

Amplification ReadyMix to supplement Extract-N-Amp Plant and Extract-N-Amp Tissue kits

Quality Level

usage

sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

feature

dNTPs included
hotstart

application(s)

agriculture

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

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This Item
P8115R4775R4526
shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

description

Amplification ReadyMix to supplement Extract-N-Amp Plant and Extract-N-Amp Tissue kits

description

-

description

-

description

-

usage

sufficient for 10000 reactions

usage

sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications

usage

sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications

usage

sufficient for ≥600 reactions

General description

Extract-N-Amp PCR ReadyMix with qPCR Reference dye is intended for use with Sigma′s Extract-N-Amp Plant kit and and Extract-N-Amp Tissue PCR kit. All kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed. Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service