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D5688

Sigma-Aldrich

Dilution Solution

Extract-N-Amp Plant Dilution Solution

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Synonym(s):
Direct PCR, Direct PCR dilution solution for plants, Extract‑N‑Amp Dilution Solution
NACRES:
NA.55

form

liquid

storage temp.

−20°C

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This Item
N9784L3289E7526
Sigma-Aldrich

Sigma-Aldrich

D5688

Dilution Solution

Sigma-Aldrich

Sigma-Aldrich

E7526

Extraction Solution

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Dilution Solution is intended for use with Sigma′s Extract-N-Amp Plant PCR Kit. Once the DNA is extracted from the leaf tissue, the Dilution Solution is added to the extract to neutralize inhibitory substances. The extract is then ready for PCR.

Application

Dilution Solution (Extract-N-Amp Plant Dilution Solution) has been used in direct PCR amplification and to prepare template DNA.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Molecular Diagnosis of Urinary Tract Infections by Semi-Quantitative Detection of Uropathogens in a Routine Clinical Hospital Setting.
van der Zee A
PLoS ONE, 11(3), e0150755-e0150755 (2016)
Anneke van der Zee et al.
BMC infectious diseases, 14, 27-27 (2014-01-16)
Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for
Anneke van der Zee et al.
BMC research notes, 4, 150-150 (2011-05-28)
To facilitate automation, a novel DNA extraction method for MRSA was adopted. The MRSA specific chromosome-SCCmec PCR was adapted, additional primers were added, and the performance was validated. From various laboratories in The Netherlands we received a total of 86
Jose L Ramirez et al.
Journal of insect science (Online), 8, 1-7 (2008-04-01)
Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), the glassy-winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei (Xanthomonadales: Xanthomonadaceae) that causes Pierce's Disease in grapevines in California. In the present study we report a new
Daniela Hegebarth et al.
Plant, cell & environment, 40(9), 1761-1776 (2017-05-10)
The aliphatic waxes sealing plant surfaces against environmental stress are generated by fatty acid elongase complexes, each containing a β-ketoacyl-CoA synthase (KCS) enzyme that catalyses a crucial condensation forming a new C─C bond to extend the carbon backbone. The relatively

Protocols

REDExtract-N-Amp™ Plant PCR Kit Protocol

The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.

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