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Sigma-Aldrich

SYBR® Green Extract-N-Amp Plant PCR Kit

sufficient for 100 preparations

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NACRES:
NA.55

usage

sufficient for 100 preparations
sufficient for 100 reactions

feature

dNTPs included
hotstart

technique(s)

qPCR: suitable

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

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This Item
XNATRGXNAS2XNAPS
usage

sufficient for 100 preparations

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 10 amplifications, sufficient for 10 extractions, sufficient for 10 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

detection method

SYBR® Green

detection method

-

detection method

-

detection method

-

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

SYBR® Green Extract-N-Amp Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.

Features and Benefits

  • Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • No loading buffers or tracking dyes required for gel analysis
  • SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Compatible with high-throughput requirements for genetic analysis of plants
  • No long enzymatic digestions
  • Extract stable at 4°C for at least 6 months

Other Notes

The protocol uses a 20 μl PCR reaction.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Kit Components Also Available Separately

Product No.
Description
SDS

  • E7526Extraction SolutionSDS

  • D5688Dilution SolutionSDS

  • S4320SYBR® Green Extract-N-Amp PCR ReadyMixSDS

related product

Product No.
Description
Pricing

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Trenis D Palmer et al.
Journal of visualized experiments : JoVE, (51)(51), doi:10-doi:10 (2011-06-16)
During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex process that can involve many tissue types, span variable time periods, and often occur deep within organs, making

Articles

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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