P6097
Precast Agarose Gels
24 wells (2 × 12-well portrait orientation)
Recommended Products
grade
for molecular biology
Quality Level
form
gel form
composition
(Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide. RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0) Quality)
storage temp.
room temp
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General description
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Well orientation: 24-well, arranged as a 2 x 12 well portrait style
Application
TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.
MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Packaging
Components
Other Notes
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)
Quality
Physical form
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)
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flash_point_f
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flash_point_c
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Certificates of Analysis (COA)
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Protocols
These gels are suitable for separating nucleic acids, giving sharp DNA bands and low background fluorescence.
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