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N1531

Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

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Synonym(s):
Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer
NACRES:
NA.25

grade

for molecular biology

form

solution

storage temp.

room temp

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This Item
D5688T9285P1415
Sigma-Aldrich

Sigma-Aldrich

N1531

Denaturation Solution

Sigma-Aldrich

Sigma-Aldrich

D5688

Dilution Solution

form

solution

form

liquid

form

liquid

form

liquid (2x concentrate)

storage temp.

room temp

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Components

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Other Notes

0.5 N NaOH, 1.5 M NaCl

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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