Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

11717472001

Roche

Hybridization Buffer

pH 6.5-6.7 (25 °C), suitable for hybridization, solution, pkg of 100 mL

Synonym(s):

Buffer

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105326

form

solution

Quality Level

100

packaging

pkg of 100 mL

manufacturer/tradename

Roche

technique(s)

hybridization: suitable

pH

6.5-6.7 (25 °C)

storage temp.

2-8°C

General description

Hybridization Buffer is a denaturing solution that optimizes the binding of DNA and RNA probes to specific target sequences in various techniques.
Hybridization buffer is used in combination with the PCR ELISA (enzyme-linked immunosorbent assay) and the series of DIG-Detection ELISAs for nucleic-acid hybridization reactions.

Application

Hybridization Buffer has been used for the prehybridization of coverslips in protein competition assay of human foreskin fibroblasts (HFF) cells. It has also been used to perform hybridization in northern blotting.

Physical form

Solution

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Fjola Rut Svavarsdottir et al.
Folia parasitologica, 68 (2021-08-18)
Proliferative kidney disease (PKD) is a widespread temperature-dependent disease in salmonids caused by the myxozoan parasite, Tetracapsuloides bryosalmonae (Canning, Curry, Feist, Longshaw et Okamura, 1999) (Tb). Tb has a two-host life cycle, involving fish as an intermediate host and freshwater
Microsporidia Interact with Host Cell Mitochondria via Voltage-Dependent Anion Channels Using Sporoplasm Surface Protein 1
Han B, et al.
mBio, 10(4), e01944-e01919 (2019)
A safer, urea-based in situ hybridization method improves detection of gene expression in diverse animal species
Sinigaglia C, et al.
Developmental Biology, 434(1), 15-23 (2018)
Microsporidia Interact with Host Cell Mitochondria via Voltage-Dependent Anion Channels Using Sporoplasm Surface Protein 1
<BIG>Han B, et al.</BIG>
mBio, 10 (2019)
Mingming Liu et al.
Nature plants, 5(4), 389-400 (2019-03-20)
Breeding crops with resistance is an efficient way to control diseases. However, increased resistance often has a fitness penalty. Thus, simultaneously increasing disease resistance and yield potential is a challenge in crop breeding. In this study, we found that downregulation

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service