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D9905

Sigma-Aldrich

Denhardt′s Solution

for Northern and Southern blotting, lyophilized powder

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Synonym(s):
Denhardt′s Solution For Blotting Technique
NACRES:
NA.25

grade

for molecular biology

form

lyophilized powder

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

2-8°C

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N1531B6429P1415
Sigma-Aldrich

Sigma-Aldrich

D9905

Denhardt′s Solution

Sigma-Aldrich

Sigma-Aldrich

N1531

Denaturation Solution

form

lyophilized powder

form

solution

form

solution

form

liquid (2x concentrate)

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

Southern blotting: suitable

technique(s)

-

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

-

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Denhardt′s Solution has been used:
  • as a component in hybridization buffer for the hybridization of brain sections
  • in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
  • in the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.

Biochem/physiol Actions

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.

Components

Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
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Alterations in m-RNA expression for Cu, Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson?s disease
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