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11796895001

Roche

DIG Easy Hyb Granules

greener alternative

powder, suitable for hybridization

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Synonym(s):
DIG system

Agency

suitable for

Quality Level

form

powder

usage

sufficient for 6 x 100 mL

manufacturer/tradename

Roche

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

hybridization: suitable

color

white

pH

7,0

solubility

water: soluble

suitability

suitable for nucleic acid hybridization

greener alternative category

storage temp.

20-25°C

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This Item
116035580011117503391011585762001
DIG Easy Hyb™ Granules powder, suitable for hybridization

Roche

11796895001

DIG Easy Hyb Granules

DIG Easy Hyb™ suitable for hybridization, solution

Roche

11603558001

DIG Easy Hyb

DIG DNA Labeling Kit sufficient for 40 labeling reactions, kit of 1 (7 components), suitable for hybridization

Roche

11175033910

DIG DNA Labeling Kit

form

powder

form

solution

form

-

form

-

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

storage condition

-

storage condition

-

storage condition

-

technique(s)

hybridization: suitable

technique(s)

hybridization: suitable

technique(s)

hybridization: suitable

technique(s)

-

color

white

color

-

color

-

color

-

pH

7,0

pH

-

pH

-

pH

-

General description

DIG Easy Hyb is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.

Features and Benefits

  • DIG Easy Hyb is a convenient ready-to-use hybridization buffer, saving time and effort.
  • DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.

Preparation Note

Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

DIG Easy Hyb is a trademark of Roche

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Protocols

Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting

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