C0712

CDP-Star^® Chemiluminescent Substrate

0.25 mM in H₂O

• Synonym(s): Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.1^3.7]decan])-4-yl]-1-phenyl phosphate
• Empirical Formula (Hill Notation): C₁₈H₁₉Cl₂O₇Na₂P
• CAS Number: 160081-62-9
• Molecular Weight: 495.20
• MDL number: MFCD02094611
• PubChem Substance ID: 329774818
• NACRES: NA.83

Properties

• Assay: ≥75% (HPLC)
• Quality Level: 200
• form: liquid
• usage: sufficient for 20 blots detection of alkaline phosphatase-labeled molecules (10 × 10 cm)
• concentration: 0.25 mM in H₂O
• storage temp.: 2-8°C
• SMILES string: Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3
• InChI: 1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;
• InChI key: UKWLRLAKGMZXJC-VCCHNUGISA-L

Description

Application

CDP-Star^® is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.

Features and Benefits

CDP-Star^® is supplied as a 0.25 mM ready-to-use aqueous solution for use in a variety of membrane-based applications. CDP-Star functions on both neutrally-charged and positively-charged nylon, giving the reagent added application versatility.

Caution

Store at 2-8 °C protected from light.

Legal Information

CDP-Star^® is a trademark of Tropix, Inc. Bedford, MA, USA and covered under U.S. Patent 5,326,882.

CDP-Star is a registered trademark of Tropix, Inc.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable