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11582950001

Roche

BM Chemiluminescence ELISA Substrate (POD)

Synonym(s):
ELISA substrate

form

solution

Quality Level

packaging

pkg of 250 mL (for 2500 wells or 1000 tubes)

manufacturer/tradename

Roche

storage temp.

2-8°C

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This Item
ELLUF01001166991500112015196001
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

packaging

pkg of 250 mL (for 2500 wells or 1000 tubes)

packaging

pkg of 100 mL

packaging

-

packaging

pkg of 100 mL (1,000 cm2 membrane)

manufacturer/tradename

Roche

manufacturer/tradename

Luminata

manufacturer/tradename

Roche

manufacturer/tradename

Roche

General description

The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.

Application

The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.

Features and Benefits

  • Improved sensitivity
  • Large dynamic range
  • Rapid and constant signal

Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H2O2)

Principle

Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.

Preparation Note

Working solution: Preparation of working solution

The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
  • Add to 100 parts of solution A one part of solution B.
  • Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.
Premixed working solution stored at 2 to 8 °C should be warmed up to 15 to 25 °C to avoid temperature effects during measurement.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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