Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

M3168

Sigma-Aldrich

4-Methylumbelliferyl phosphate

alkaline phosphatase substrate, liquid

Synonym(s):

4-methylumbelliferone phosphate Liquid Substrate System

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
3368-04-5
EC Number:
222-137-7
MDL number:
MFCD00016969
UNSPSC Code:
12352204
PubChem Substance ID:
24896808
NACRES:
NA.32

product name

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid

form

liquid

Quality Level

200

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

InChI key

BCHIXGBGRHLSBE-UHFFFAOYSA-N

General description

4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.

Application

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.

Analysis Note

4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Modification of pasta structure induced by high drying temperatures. Effects on the in vitro digestibility of protein and starch fractions and the potential allergenicity of protein hydrolysates
Petitot M, et al.
Food Chemistry, 116(2), 401-412 (2009)
Assessment of allergenicity of diploid and hexaploid wheat genotypes: Identification of allergens in the albumin/globulin fraction
Larre C, et al.
Journal of Proteomics, 74(8), 1279-1289 (2011)
Activation of NF-kappaB by rank requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase identification of a novel TRAF6 interaction motif
Darnay BG, et al.
The Journal of biological chemistry, 274(12), 7724-7731 (1999)
Christopher P Holmes et al.
Bioorganic & medicinal chemistry letters, 18(11), 3382-3385 (2008-04-30)
As part of a program to develop better PTP1B fluorogenic substrates that more closely mimic the functionality found in the natural substrate, we have prepared and evaluated nine novel analogs of 4-methylumbelliferone phosphate (MUP) with a variety of additional groups
Degree of hydrolysis and antigenicity of buffalo alpha S1 casein and its hydrolysates in children with cow milk allergy
Ahmad N, et al.
Food and agricultural immunology, 27(1), 87-98 (2016)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service