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APF

Sigma-Aldrich

Alkaline Phosphatase Detection Kit, Fluorescence

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About This Item

EC Number:
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.53

grade

for molecular biology

Quality Level

usage

 kit sufficient for 300 reactions (200 μl reactions)

technique(s)

microbe id | specific enzyme detection: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ALPL(249)

General description

The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.

Application

Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity.
Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.

Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Biochem/physiol Actions

Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.

Features and Benefits

  • Up to 100x more sensitive than colorimetric assays
  • SEAP can be measured without sample destrutcion
  • Rapid and reproducible results
  • Safe and easy-to-use

Components

The Alkaline Phosphatase Kit contains:
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Caterina Bossio et al.
Frontiers in bioengineering and biotechnology, 6, 114-114 (2018-09-14)
Optical modulation of living cells activity by light-absorbing exogenous materials is gaining increasing interest, due to the possibility both to achieve high spatial and temporal resolution with a minimally invasive and reversible technique and to avoid the need of viral
Irena Moserova et al.
PloS one, 7(3), e32972-e32972 (2012-03-10)
We have analyzed the molecular mechanisms of photoinduced cell death using porphyrins with similar structure differing only in the position of the ethylene glycol (EG) chain on the phenyl ring. Meta- and para-positioned EG chains targeted porphyrins to different subcellular
Darren M Brey et al.
Journal of biomedical materials research. Part A, 93(2), 807-816 (2010-03-04)
Combinatorial polymer syntheses are now being utilized to create libraries of materials with potential utility for a wide variety of biomedical applications. We recently developed a library of photopolymerizable and biodegradable poly(beta-amino ester)s (PBAEs) that possess a range of tunable
Kristen L Lee et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(3), 1157-1165 (2013-11-28)
Primary cilia are single, nonmotile, antenna-like structures extending from the apical membrane of most mammalian cells. They may mediate mechanotransduction, the conversion of external mechanical stimuli into biochemical intracellular signals. Previously we demonstrated that adenylyl cyclase 6 (AC6), a membrane-bound
An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu 2+ bound to poly-thymine DNA
Lee JY, et al.
Royal Society of Chemistry Advances, 8(20), 11241-11246 (2018)

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