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Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast red violet LB

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UNSPSC Code:
12352106
eCl@ss:
42010105
NACRES:
NA.47

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast red violet LB. The resulting insoluble diffuse, red dye deposit indicates sites of alkaline phosphatase activity.

Kit Components Only

Product No.
Description

  • Citrate Solution (915) 50 mL

  • FRV-Alkaline Solution (862) 10 mL

  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL

  • Naphthol AS-BI Alkaline Solution (861) 10 mL

  • Sodium Nitrite Solution (914) 10 mL

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with
Clive H Glover et al.
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Stem cell differentiation involves critical changes in gene expression. Identification of these should provide endpoints useful for optimizing stem cell propagation as well as potential clues about mechanisms governing stem cell maintenance. Here we describe the results of a new

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