All Photos(1)




Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

10x Blocking Buffer


for molecular biology

Quality Level



storage temp.

room temp

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item



5% Alkali-soluble Casein









storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.


General description

Sigma′s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.


Casein Blocking Buffer 10x has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.


Exclamation markEnvironment

Signal Word


Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Anna Ah-San Tang et al.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
Chadwick M Hales et al.
Proteomics, 16(23), 3042-3053 (2016-10-09)
Despite a key role of amyloid-beta (Aβ) in Alzheimer's disease (AD), mechanisms that link Aβ plaques to tau neurofibrillary tangles and cognitive decline still remain poorly understood. The purpose of this study was to quantify proteins in the sarkosyl-insoluble brain
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)


Casein Blocking Buffer Protocol for Southern or Western Blotting

Protocol for using Casein Blocking Buffer in Southern and Western blotting applications, including colorimetric and chemiluminescent substrate detection options. Troubleshooting for optimizing signal and reducing background is also included.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service