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B6429

Sigma-Aldrich

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

All Photos(1)
Synonym(s):
10x Blocking Buffer

grade

for molecular biology

Quality Level

200

form

solution

storage temp.

room temp

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This Item
B6185S201570955
Sigma-Aldrich

Sigma-Aldrich

B6429

Casein Blocking Buffer 10x

Bionic™ Buffer, 10X Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

B6185

Bionic™ Buffer, 10X Concentrate

Sigma-Aldrich

Sigma-Aldrich

S2015

SSPE Buffer 20× Concentrate

Millipore

Millipore

70955

5% Alkali-soluble Casein

form

solution

form

solution

form

solution

form

liquid

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

General description

Sigma′s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315 - H317 - H319 - H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

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Protocols

Casein Blocking Buffer Protocol for Southern or Western Blotting

Protocol for using Casein Blocking Buffer in Southern and Western blotting applications, including colorimetric and chemiluminescent substrate detection options. Troubleshooting for optimizing signal and reducing background is also included.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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