MilliporeSigma
All Photos(1)

Documents

W0138

Sigma-Aldrich

Western Blocker™ Solution

for HRP detection systems

All Photos(1)
Synonym(s):
Western blot blocking solution, blocking buffer
NACRES:
NA.25

Quality Level

200

shelf life

≥2 yr

storage temp.

2-8°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
2504GBL105101G7663
Sigma-Aldrich

Sigma-Aldrich

W0138

Western Blocker™ Solution

Millipore

Millipore

2504

ReBlot Plus Strong Antibody Stripping Solution, 10x

Grace Bio-Labs Super G blocking buffer volume 500 mL, sterile

GBL105101

Grace Bio-Labs Super G blocking buffer

Sigma-Aldrich

Sigma-Aldrich

G7663

Gelatin blocking buffer

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

room temp

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

General description

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Application

Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.

Features and Benefits

  • Does not inhibit weak HRP signals, as many milk-based blockers do.
  • Can be used both as a blocking component and as a probing component.
  • Supplied ready-to-use; no preparative step is required.

Legal Information

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

In vitro differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells.
Pournasr B, et al.
Archives of Iranian Medicine, 14, 244-249 (2011)
A N Meleshko et al.
Experimental oncology, 35(1), 8-14 (2013-03-27)
Idiotype, the unique part of immunoglobulin molecule expressed on the surface of B-cells, represents a specific antigen for vaccination against lymphoma. We have developed a rapid method for immunoglobulin variable fragments cloning, assembling and expression of recombinant idiotype protein in
Massimo Conese et al.
International journal of molecular sciences, 21(12) (2020-06-26)
Recently, insulin-like growth factor binding protein 6 (IGFBP-6) has been shown to play a putative role in the immune system, as monocyte-derived dendritic cells (Mo-DCs) are stimulated by hyperthermia to express IGFBP-6 at both the mRNA and protein levels. However
Lalit P Chandravanshi et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 34, 60-75 (2014-02-13)
In view of the increasing incidences of arsenic induced health effects and the vulnerability of the developing brain to its toxic effects, studies have been carried out to investigate the mechanism of arsenic induced cholinergic alterations and understand if such
Ayça Fulya Üstüntanır Dede et al.
Gene, 748, 144697-144697 (2020-04-24)
Artificial chromosomes were previously generated for use in bacteria, protists, yeast and human cells. A Tetrahymena thermophila artificial chromosome could serve as a versatile platform to study diverse aspects of Tetrahymena biology and beyond. Here, we placed a C3-type rDNA
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service