WESTBL-RO
Roche
Western Blocking Reagent, Solution
solution, sufficient for 10 blots (11921673001 [100 cm2]), sufficient for 60 blots (11921681001 [100 cm2])
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blocking reagent for western blots
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form
solution
Quality Level
usage
sufficient for 10 blots (11921673001 [100 cm2])
sufficient for 60 blots (11921681001 [100 cm2])
packaging
pkg of 100 mL (11921673001)
pkg of 6 × 100 mL (11921681001)
manufacturer/tradename
Roche
storage temp.
2-8°C
Related Categories
Application
Western Blocking Reagent is used as a general blocking agent during western blotting experiments and immunofluorescence staining. The reagent is used in steps such as blocking the membrane, membrane washing steps, and diluting detection antibodies.
Quality
Each lot is function tested (dot blot).
Physical form
The Western Blocking Reagent is a 10x solution that contains 10% purified casein protein in maleic-acid buffer.
Preparation Note
Working solution: PREPARATION OF WORKING SOLUTIONS
Note: Since sodium azide inhibits POD, it must not be used as antimicrobial agent when using POD-conjugates.
Note: 0.1% Tween 20 is suitable for the most applications, but—depending on the membrane and on the antibody used—different detergents (like SDS, Triton X-100 and Nonidet P40 and detergent concentrations from 0.01 to 1% may lead to better results.
Note: Since sodium azide inhibits POD, it must not be used as antimicrobial agent when using POD-conjugates.
Note: The concentration of the blocking reagent is an important parameter for improvement of the signal to noise ratio in Western blots. If high background appears even under optimized antibody concentrations, increase the concentration of the blocking reagent during the antibody incubations and washing steps from 0.5% to 1%. In case of weak signals even with prolonged antibody incubations lower the concentration of blocking reagent during the antibody incubations and washing steps from 0.5% to 0.1%.
- Tris buffered saline (TBS), pH 7.5
Note: Since sodium azide inhibits POD, it must not be used as antimicrobial agent when using POD-conjugates.
- TBS-Tween (TBST)
Note: 0.1% Tween 20 is suitable for the most applications, but—depending on the membrane and on the antibody used—different detergents (like SDS, Triton X-100 and Nonidet P40 and detergent concentrations from 0.01 to 1% may lead to better results.
- Blocking solution (1%)
Note: Since sodium azide inhibits POD, it must not be used as antimicrobial agent when using POD-conjugates.
- Blocking solution (0.5%)
- Antibody solutions
Note: The concentration of the blocking reagent is an important parameter for improvement of the signal to noise ratio in Western blots. If high background appears even under optimized antibody concentrations, increase the concentration of the blocking reagent during the antibody incubations and washing steps from 0.5% to 1%. In case of weak signals even with prolonged antibody incubations lower the concentration of blocking reagent during the antibody incubations and washing steps from 0.5% to 0.1%.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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