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T7777

Sigma-Aldrich

Tris-Glycine-SDS Buffer 10× Concentrate

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Synonym(s):
TRIS glycine SDS buffer solution
eCl@ss:
32129211
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

form

liquid

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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1 of 4

This Item
T1165SRE0068T9285
vibrant-m

SRE0068

SSC Buffer, 20X Concentrate

vibrant-m

T9285

Tris-EDTA buffer solution

form

liquid

form

liquid

form

liquid

form

liquid

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

0.2 μm filtered

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

sample preparation

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

General description

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Application

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Reconstitution

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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