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P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid

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Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
MDL number:
PubChem Substance ID:

product line

BioReagent

Quality Level

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

density

1.01 g/cm3 at 20 °C

suitability

suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

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This Item
P3504141194318744
Ponceau S solution BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid

Sigma-Aldrich

P7170

Ponceau S solution

Ponceau S BioReagent, suitable for electrophoresis

Sigma-Aldrich

P3504

Ponceau S

Ponceau S Dye content 75 %

Sigma-Aldrich

141194

Ponceau S

form

liquid

form

powder

form

powder

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

concentration

-

concentration

-

concentration

0.04 wt. % in H2O

density

1.01 g/cm3 at 20 °C

density

-

density

-

density

0.985 g/mL at 25 °C

suitability

suitable for electrophoresis

suitability

-

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

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