31149
Deoxyribonucleic acid, low molecular weight from salmon sperm
Synonym(s):
Salmon sperm dsDNA, low molecular weight DNA, DNA
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About This Item
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grade
for molecular biology
puriss. p.a.
form
lyophilized powder
impurities
≤5% protein
solubility
50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
absorbance ratio
A260/280 nm ~1.4
storage temp.
2-8°C
Application
Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
- to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.
- in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.
- as a precursor in the synthesis of the bio-dots.
Biochem/physiol Actions
Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.
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Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
Bioelectrochemistry (Amsterdam, Netherlands), 116, 1-9 (2017-03-18)
A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer
Thiol modification of silicon-substituted hydroxyapatite nanocrystals facilitates fluorescent labelling and visualisation of cellular internalisation
Journal of Material Chemistry B: Materials for Biology and Medicine, 35, 4370-4378 (2013)
Biochemistry. Biokhimiia, 74(11), 1285-1288 (2009-11-18)
A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters
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