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N0505

Sigma-Aldrich

β-Nicotinamide adenine dinucleotide phosphate sodium salt hydrate

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Synonym(s):
Triphosphopyridine nucleotide sodium salt hydrate
Empirical Formula (Hill Notation):
C21H27N7NaO17P3 · xH2O
CAS Number:
Molecular Weight:
765.39 (anhydrous basis)
MDL number:
PubChem Substance ID:
NACRES:
NA.51

assay

≥98% (HPLC)
≥98% (spectrophotometric assay)

Quality Level

form

powder

mp

175-178 °C (dec.) (lit.)

solubility

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

O[C@H]1[C@@H](O)[C@H]([N+]2=CC(C(N)=O)=CC=C2)O[C@@H]1COP(OP(OC[C@H]3O[C@@H](N(C=N4)C5=C4C(N)=NC=N5)[C@H](OP(O)(O)=O)[C@@H]3O)([O-])=O)([O-])=O.O.[Na+]

InChI

1S/C21H28N7O17P3.Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39);;1H2/q;+1;/p-1/t10-,11-,13-,14-,15-,16-,20-,21-;;/m1../s1

InChI key

CYQLUFJYVTUUFN-WUEGHLCSSA-M

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1 of 4

This Item
N3139N3886N7505
Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

300

assay

≥98% (HPLC), ≥98% (spectrophotometric assay)

assay

≥98% (HPLC), ≥98% (spectrophotometric assay)

assay

≥95% (HPLC), ≥95% (spectrophotometric assay)

assay

≥97% (HPLC), ≥97% (spectrophotometric assay)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

powder

form

powder

form

powder

form

powder

mp

175-178 °C (dec.) (lit.)

mp

175-178 °C (dec.) (lit.)

mp

175-178 °C (dec.) (lit.)

mp

-

Biochem/physiol Actions

Electron acceptor

Packaging

Packaged by solid weight.

Other Notes

This is the common form of NADP. Do not confuse with 3′−NADP or α-NADP.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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F Moreno et al.
European journal of biochemistry, 161(3), 565-569 (1986-12-15)
Hexokinase PII is not inhibited by high Mg-ATP concentrations if the Mg2+-free ATP is kept at low levels (0.01 mM) in the assay mixture. Hexokinase PI activity is not affected either by Mg2+-free ATP nor by free Mg2+ in the
Syaghalirwa N M Mandiki et al.
Steroids, 70(2), 85-94 (2005-01-06)
Sex steroid inhibitors were used to characterize the effects of 17beta-estradiol (E2) and testosterone (T) on the sexual growth dimorphism of Eurasian perch juveniles. In experiment 1, growth responses to different doses of either E2 (25, 50, 75, and 100
David J Waxman et al.
Methods in molecular biology (Clifton, N.J.), 320, 91-96 (2006-05-25)
A spectrofluorometric method is described for the determination of CYP2A6-catalyzed coumarin 7-hydroxylation. Following acidification of the reaction mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-extraction procedure and assayed using an excitation wavelength of 370 nm and an emission
Considine, DM et al.
Van Nostrand Reinhold Encyclopedia of Chemistry, 1993-1993 null
J T Eppig et al.
Genetics, 103(4), 797-812 (1983-04-01)
Murine ovarian teratomas were used to determine recombination percentages for gene-gene and centromere-gene intervals. Data were obtained utilizing a recombinant inbred strain, LTXBJ, and a number of newly developed LT/SvEi congenic strains.--Centromere-gene recombination was measured at 11.3 +/- 1.2% for

Protocols

To measure hexokinase activity, a continuous spectrophotometric rate-determination assay is used at 340 nm. Hexokinase catalyzes the phosphorylation of D-hexose sugars using ATP as the phosphate source.

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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