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G7877

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

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Synonym(s):
glucose-6-phosphate dehydrogenase (NADP+), G-6-P-DH, Zwischenferment
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bakers yeast

Quality Level

type

Type VII

form

ammonium sulfate suspension

specific activity

≥200 units/mg protein

mol wt

128 kDa

technique(s)

cell based assay: suitable

solubility

water: soluble

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

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This Item
G5885G8404G8529
form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

specific activity

≥200 units/mg protein

specific activity

>= 550 units/mg protein (biuret)

specific activity

≥550 units/mg protein (biuret)

specific activity

≥550 units/mg protein (biuret)

mol wt

128 kDa

mol wt

128 kDa

mol wt

128 kDa

mol wt

-

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

application(s)

diagnostic assay manufacturing

application(s)

agriculture

application(s)

-

application(s)

agriculture

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 7.0

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Alpha-ketoglutarate partially alleviates effects of high-fat high-fructose diet in mouse muscle
Vatashchuk MV, et al.
EXCLI Journal , 1264-1277 (2023)
Gang Zhao et al.
Neurochemistry international, 61(2), 146-155 (2012-05-15)
NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD
Xuehuai Shen et al.
General and comparative endocrinology, 177(1), 177-186 (2012-04-03)
Dehydroepiandrosterone (DHEA), a major steroid hormone, decreases with age, and this reduction has been shown to be associated with physical health. In the present study, the effect of DHEA on cell growth and mitochondrial function was investigated using TM-3 cells
Samantha R Soncini et al.
PLoS genetics, 16(7), e1008484-e1008484 (2020-07-17)
Yeast and fast-growing human tumor cells share metabolic similarities in that both cells use fermentation of glucose for energy and both are highly sensitive to the glucose analog 2-deoxyglucose. Spontaneous mutations in S. cerevisiae that conferred resistance to 2-deoxyglucose were
Chapter 39 - Personalized Medicine in Cancer Treatment
V.M. Pratt and S.A. Scott
Diagnostic Molecular Pathology, 503-513 (2017)

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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