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G7877

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

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Synonym(s):
glucose-6-phosphate dehydrogenase (NADP+), G-6-P-DH, Zwischenferment
CAS Number:
9001-40-5
Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
EC Number:
232-602-6
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bakers yeast

Quality Level

200

type

Type VII

form

ammonium sulfate suspension

specific activity

≥200 units/mg protein

mol wt

128 kDa

technique(s)

cell based assay: suitable

solubility

water: soluble

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Gene Information

Saccharomyces cerevisiae ... ZWF1(855480)

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General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Application

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 7.0

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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