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S8140

Sigma-Aldrich

SSPE Buffer

for Northern and Southern blotting, powder blend

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NACRES:
NA.25

grade

for molecular biology

form

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

solubility

water, high purity: 20 ×, clear, colorless

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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This Item
S2015S663993017
vibrant-m

S8140

SSPE Buffer

vibrant-m

S2015

SSPE Buffer 20× Concentrate

vibrant-m

S6639

SSC Buffer 20× Concentrate

vibrant-m

93017

SSC Buffer 20x

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

solubility

water, high purity: 20 ×, clear, colorless

solubility

-

solubility

-

solubility

-

form

powder blend

form

solution

form

solution

form

solution

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

foreign activity

DNAse, none detected, Exonuclease, none detected, RNAse, none detected, Endonuclease, none detected, NICKase, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

General description

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Features and Benefits

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Components

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
Igor V. Kutyavin
Nucleic Acids Research, 28(2) (2000)
Microfluidic-based enzymatic on-chip labeling of miRNAs
Sonja Vorwek
New Biotechnology (2008)

Protocols

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service