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PPB021

Sigma-Aldrich

SSPE Buffer

pH 7.4, pHast Pack, powder

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Synonym(s):
1X SSPE Hybridization buffer, 1X SSPE buffer, SSPE Hybridization buffer, Saline-Sodium Phosphate-EDTA Buffer

product line

pHast Pack

Quality Level

form

powder

pH

7.4

Related Categories

Application

Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.

Features and Benefits

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Save time and minimize effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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