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P6222

Sigma-Aldrich

Precast Agarose Gels

8-well (portrait orientation)

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Synonym(s):
8-well Precast Agarose Gels, Precast agarose gels for nucleic acid separation
NACRES:
NB.22

grade

for molecular biology

Quality Level

form

gel form

does not contain

ethidium bromide

concentration

1.25% agarose (in 1x MOPS buffer; for RNA electrophoresis)

storage temp.

room temp

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This Item
P5472GE17-0554-0211696505001
Precast Agarose Gels 8-well (portrait orientation)

Sigma-Aldrich

P6222

Precast Agarose Gels

Precast Agarose Gels 8-well (portrait orientation)

Sigma-Aldrich

P5472

Precast Agarose Gels

Agarose NA Cytiva 17-0554-02, pack of 100 g

GE17-0554-02

Agarose NA

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

form

gel form

form

gel form

form

solid

form

-

does not contain

ethidium bromide

does not contain

-

does not contain

-

does not contain

-

concentration

1.25% agarose (in 1x MOPS buffer; for RNA electrophoresis)

concentration

-

concentration

-

concentration

-

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

General description

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style

Application

Precast Agarose Gels has been used for the electrophoresis of purified PCR products.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.

Packaging

Package of 20 individually foil-wrapped gels.

Other Notes

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

Quality

No detected DNase or RNase activity

Physical form

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T1503
Product Number
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25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Nikolai A Shevchuk et al.
Nucleic acids research, 32(2), e19-e19 (2004-01-24)
A procedure for precise assembly of linear DNA constructs as long as 20 kb is proposed. The method, which we call long multiple fusion, has been used to assemble up to four fragments simultaneously (for a 10.8 kb final product)
Claudia S Meier-Trummer et al.
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Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease

Protocols

Precast Agarose Gels for RNA Electrophoresis

Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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