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SYBR® Green Quantitative RT-qPCR Kit

One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

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Synonym(s):
one step rt qPCR, sybr green qPCR
NACRES:
NA.55

Quality Level

usage

sufficient for 250 reactions

feature

dNTPs included
hotstart

technique(s)

RT-qPCR: suitable

color

colorless

input

purified RNA

compatibility

ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

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This Item
S9194KCQS00KCQS01
usage

sufficient for 250 reactions

usage

sufficient for 20 reactions, sufficient for 2000 reactions, sufficient for 400 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

colorless

color

colorless

color

colorless

color

colorless

input

purified RNA

input

purified DNA

input

purified DNA

input

purified DNA

compatibility

ABI 5700

compatibility

for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus

compatibility

for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480

compatibility

for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000

General description

The SYBR® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

SYBR® Green Quantitative RT-qPCR Kit has been used in a 1-step reverse transcription polymerase chain reaction (RT-PCR) assay:
  • to detect specific genetic clusters of genogroup I and II noroviruses
  • for chikungunya viral (CHIKV) RNA quantification
  • to detect mRNA expression levels
  • for amplification of total RNA extracted from human umbilical vein endothelial cells (HUVECs) and prostate cancer cells

Features and Benefits

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced preparation time and the risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStartTaq Polymerase reduces primer-dimer and non-specific product formation
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 100 reactions at 50 μL each

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Kit Components Only

Product No.
Description

  • SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25

Kit Components Also Available Separately

Product No.
Description
SDS

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 includedSDS

  • M878725 mM MgCl2 1.5 mL/vialSDS

  • R4526Reference Dye for Quantitative PCR, 100 ×, solution .3 mL/vialSDS

related product

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Carmela R Balistreri et al.
Scientific reports, 9(1), 11028-11028 (2019-08-01)
Bicuspid aortic valve (BAV) disease is recognized to be a syndrome with a complex and multifaceted pathophysiology. Its progression is modulated by diverse evolutionary conserved pathways, such as Notch-1 pathway. Emerging evidence is also highlighting the key role of TLR4
Pei Jin Lim et al.
PLoS neglected tropical diseases, 8(2), e2661-e2661 (2014-03-04)
Chikungunya virus (CHIKV) has resulted in several outbreaks in the past six decades. The clinical symptoms of Chikungunya infection include fever, skin rash, arthralgia, and an increasing incidence of encephalitis. The re-emergence of CHIKV with more severe pathogenesis highlights its
Caiyun Shan et al.
Molecular medicine reports, 17(4), 5300-5305 (2018-02-03)
Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA‑126 (miR‑126)‑associated signaling pathways
Phui San Ho et al.
Virology journal, 7, 13-13 (2010-01-23)
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus and one of the prevalent re-emerging arbovirus in tropical and subtropical regions of Asia, Africa, and Central and South America. It produces a spectrum of illness ranging from inapparent infection to moderate febrile
Gary P Richards et al.
Applied and environmental microbiology, 70(12), 7179-7184 (2004-12-03)
Genogroup I noroviruses from five genetic clusters and genogroup II noroviruses from eight genetic clusters were detected in stool extracts using degenerate primers and single-tube, real-time reverse transcription-PCR (RT-PCR) with SYBR Green detection. Two degenerate primer sets, designated MON 431-433

Articles

RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Protocols

Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Related Content

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

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