Documents
Recommended Products
usage
sufficient for 200 reactions
sufficient for 2000 reactions
Quality Level
feature
dNTPs included: no
hotstart
manufacturer/tradename
Roche
packaging
pkg of 200 x 50 μL reactions (04913850001)
pkg of 2000 x 50 μL reactions (04913914001)
technique(s)
RT-qPCR: suitable
qPCR: suitable
input
purified DNA
detection method
probe-based
Related Categories
1 of 4
This Item | L6544 | FSUPMMRO | KCQS00 |
|---|---|---|---|
| usage sufficient for 200 reactions | usage sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions |
| feature dNTPs included: no | feature dNTPs included, hotstart | feature dNTPs included: no, hotstart | feature dNTPs included, hotstart |
| manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename - |
| packaging pkg of 200 x 50 μL reactions (04913850001) | packaging - | packaging pkg of 1250 x 20 μL reactions (04913957001), pkg of 250 x 20 μL reactions (04913949001), pkg of 5000 x 20 μL reactions (04914058001) | packaging - |
| input purified DNA | input purified DNA | input purified DNA | input purified DNA |
General description
SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, which is included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature (+15 to +25°C). Therefore, there is no elongation during the period when primers can non-specifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
Application
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
FastStart™ Universal SYBR® Green Master (Rox) has been used in qRT-PCR and qPCR
Features and Benefits
- Improve PCR sensitivity and specificity.
- Avoid over-estimation of qPCR results.
- Amplify and detect a broad range of DNA or cDNA targets.
- Save time and effort in qPCR preparation.
- Prevent false positives resulting from carryover contamination.
Components
Quality
Other Notes
Legal Information
also commonly purchased with this product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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Articles
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Related Content
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
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