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FastStart Universal SYBR Green Master (Rox)

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

usage

sufficient for 200 reactions
 sufficient for 2000 reactions

Quality Level

100

feature

dNTPs included: no
hotstart

manufacturer/tradename

Roche

packaging

pkg of 200 x 50 μL reactions (04913850001)
pkg of 2000 x 50 μL reactions (04913914001)

technique(s)

RT-qPCR: suitable
qPCR: suitable

input

purified DNA

detection method

probe-based

General description

FastStart™ Universal SYBR® Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.

SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, which is included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature (+15 to +25°C). Therefore, there is no elongation during the period when primers can non-specifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).

Application

FastStart™ Universal SYBR® Green Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich.
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
FastStart™ Universal SYBR® Green Master (Rox) has been used in qRT-PCR and qPCR

Features and Benefits

  • Improve PCR sensitivity and specificity.
Minimize the formation of nonspecific amplification products.

  • Avoid over-estimation of qPCR results.
Eliminate nonspecific amplification products and primer-dimers that increase the amount of bound quantified SYBR Green I.
  • Amplify and detect a broad range of DNA or cDNA targets.
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.

  • Save time and effort in qPCR preparation.
Eliminate the need to mix components, titrate MgCl2, or perform other time-consuming optimization steps.

  • Prevent false positives resulting from carryover contamination.
Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

Components

FastStart Universal SYBR Green Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), SYBR Green I, and a reference dye.

Quality

Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (approximately 440 bp).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

FastStart is a trademark of Roche
SYBR is a registered trademark of Life Technologies

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

How qPCR Works

Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

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PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

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