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G2N350

Sigma-Aldrich

GenElute Plant Genomic DNA Miniprep Kit

greener alternative

sufficient for 350 purifications

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Synonym(s):
Plant Genomic DNA Miniprep, Gen Elute
NACRES:
NA.55

usage

sufficient for 350 purifications

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

technique(s)

DNA purification: suitable

greener alternative category

storage temp.

15-25°C

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This Item
G2N10G1N350G1N70
usage

sufficient for 350 purifications

usage

sufficient for 10 purifications

usage

sufficient for 350 purifications

usage

sufficient for 70 purifications

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

-

G2N10

G2N350, G2N70

G1N10, G1N70, G1N9602, G1N9604

G1N10, G1N350, G1N9602, G1N9604

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

-

greener alternative category

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots

Features and Benefits

  • Starting material: Up to 100 mg of plant tissue
  • Expected yield: Up to 20 μg
  • Elution volume: 100 - 200 μl
  • Time required: < 40 min
  • RNase treatment required: No

Principle

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2112Column Preparation SolutionSDS

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2


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Protocols

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service