P8169
Phytagel™
BioReagent, suitable for plant cell culture, powder
Synonym(s):
Gellan Gum, Agar substitute gelling agent
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About This Item
Recommended Products
product line
BioReagent
Quality Level
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
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General description
Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.
Application
Phytagel™ has been used:
- in the solidification of culture medium for root assays in Arabidopsis thaliana
- as a component of Solid Paul′s medium for Agrobacterium culture
- as a component of root induction medium in Ilex dumosa tissue culture
Biochem/physiol Actions
Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
Other Notes
An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
Reconstitution
To prevent clumping, add slowly to rapidly stirring medium prior to heating.
Legal Information
Phytagel is a trademark of Sigma-Aldrich Co. LLC
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
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