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M0404

Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

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Synonym(s):
MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium
MDL number:
NACRES:
NA.72

Quality Level

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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This Item
M9274M5519K4003
vibrant-m

M0404

Murashige and Skoog Basal Medium

vibrant-m

M9274

Murashige and Skoog Basal Medium

vibrant-m

M5519

Murashige and Skoog Basal Medium

vibrant-m

K4003

Knudson C Modified Orchid Medium

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

-

Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies
  • in the suspension culture of Nicotiana plumbaginifolia for transformation
  • in the root culture of hairy clone A. artemisiifolia T4

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

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Warning

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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