G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Synonym(s):
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
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form
fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
- as a component of solid GM medium for germination of Arabidopsis seeds
Formula variant
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
Quantity
Formulated to contain 3.2 grams of powder per liter of medium.
signalword
Warning
hcodes
Hazard Classifications
Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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