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G5893

Sigma-Aldrich

Gamborg′s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

Synonym(s):

B-5 Basal Medium, Basal Medium, Minimal Organics Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

fine powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

General description

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Application

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
  • as a component of liquid media for the tissue culture of Brassica rapa
  • as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
  • as a component of solid GM medium for germination of Arabidopsis seeds

Formula variant

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.2 grams of powder per liter of medium.

pictograms

Flame over circle
GHS03

signalword

Warning

hcodes

H272

Hazard Classifications

Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Experiments in Plant Tissue Culture (1985)
Jakob Petereit et al.
Frontiers in plant science, 8, 72-72 (2017-02-24)
In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown
Martin E Kolewe et al.
Plant cell reports, 29(5), 485-494 (2010-03-11)
Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus
Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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