MilliporeSigma
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XNAP2E

Sigma-Aldrich

Extract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 500 amplifications

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Synonym(s):
Plant direct PCR kit
NACRES:
NA.55

usage

sufficient for 100 extractions
sufficient for 500 amplifications
sufficient for 500 reactions

feature

dNTPs included
hotstart

technique(s)

PCR: suitable

color

colorless

shipped in

wet ice

storage temp.

−20°C

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This Item
XNAPXNAPRXNAPS
usage

sufficient for 100 extractions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

usage

sufficient for 10 amplifications, sufficient for 10 extractions, sufficient for 10 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

colorless

color

red

color

red

color

red

XNAP2

XNAPE, XNAPR, XNAPS, microbialanalysis
microbialanalysis

XNAP, XNAPE, XNAPS, microbialanalysis
microbialanalysis

XNAP, XNAPE, XNAPR, microbialanalysis
microbialanalysis

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

General description

The Extract-N-Amp Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
The PCR reaction mix facilitates amplification directly from the extract by using an antibody-based hot-start DNA polymerase for specific amplification.

Application

Extract-N-Amp Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).

Extract-N-Amp Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Features and Benefits

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Components

Extract-N-Amp Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
Extraction Solution- E7526-12 mL

Dilution Solution- D5688-12mL

Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml

Collection tubes

Other Notes

The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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S A Weller et al.
Journal of microbiological methods, 70(2), 379-383 (2007-06-26)
Real-time (TaqMan) PCR assays were developed to detect the strawberry angular leaf spot pathogen Xanthomonas fragariae (Xf) and the strawberry bacterial blight pathogen Xanthomonas arboricola pv. fragariae (Xaf). The Xf PCR (Xf gyrB) was designed within regions of the gyraseB
Len N Gillman et al.
Journal of evolutionary biology, 23(6), 1327-1330 (2010-05-12)
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer
Sophie Lev et al.
Molecular plant-microbe interactions : MPMI, 22(9), 1093-1103 (2009-08-07)
Phosphorylated mitogen-activated protein kinases (MAPK) transmit signals by activation of their targets. The extent of signal transduction could depend on MAPK phosphorylation level, concentration, and subcellular localization. The pathogenicity MAPK Chk1 of the fungal corn pathogen Cochliobolus heterostrophus is required
Noa Eliahu et al.
Eukaryotic cell, 6(3), 421-429 (2007-01-24)
The maize pathogen Cochliobolus heterostrophus requires two mitogen-activated protein kinases (MAPKs), Chk1 and Mps1, to produce normal pigmentation. Young colonies of mps1 and chk1 deletion mutants have a white and autolytic appearance, which was partially rescued by a hyperosmotic environment.
Rena Shimizu et al.
Plant physiology, 149(2), 841-850 (2008-12-17)
The WUSCHEL-related homeobox (WOX) gene PRESSED FLOWER1 (PRS1) performs a conserved function during lateral organ development in Arabidopsis (Arabidopsis thaliana). Expressed in the periphery of the shoot meristem, PRS1 recruits founder cells that form lateral domains of vegetative and floral

Articles

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service