Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

PMID25473981

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

13-1920

Sigma-Aldrich

Hydrogen peroxide solution, SAJ first grade, ≥30.0%

16911

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

95294

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

44890

Sigma-Aldrich

Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

316989

Sigma-Aldrich

Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis

18304

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

IRMM524B

Iron, IRMM^®, certified reference material, 0.5 mm wire

356808

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

209309

Sigma-Aldrich

Iron, powder, −325 mesh, 97%

267945

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

95321

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

F8263

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

F8633

Sigma-Aldrich

Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

H1009

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

GF23776577

Iron, foil, light tested, 25x25mm, thickness 0.05mm, hard, 99.5%

GF33231923

Iron, foil, 6mm disks, thickness 0.5mm, hard, 99.5%

GF74718030

Iron, foil, 50mm disks, thickness 0.25mm, hard, 99.5%

GF42948771

Iron, foil, light tested, 100x100mm, thickness 0.025mm, as rolled, 99.99+%

GF37828907

Iron, foil, 8mm disks, thickness 0.05mm, as rolled, 99.99+%

GF12758834

Iron, foil, 6mm disks, thickness 0.125mm, hard, 99.5%

GF18004115

Iron, foil, not light tested, 100x100mm, thickness 0.005mm, 99.85%

GF73036795

Iron, foil, 50mm disks, thickness 0.38mm, hard, 99.5%

GF93388999

Iron, foil, light tested, 25x25mm, thickness 0.075mm, hard, 99.5%

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Iron, foil, 6mm disks, thickness 0.020mm, 99.85%

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Iron, foil, not light tested, 100x100mm, thickness 0.007mm, 99.85%

GF99169554

Iron, foil, 6mm disks, thickness 0.007mm, 99.85%