Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

PMID25473981

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

IRMM524B

Iron, IRMM^®, certified reference material, 0.5 mm wire

95321

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

18304

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

267945

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

356808

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

95294

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

316989

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Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis

44890

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Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

209309

Sigma-Aldrich

Iron, powder, −325 mesh, 97%

H1009

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Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

F8633

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Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

13-1920

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15-1490

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215422

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16911

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

88597

Millipore

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F8263

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

323381

Sigma-Aldrich

Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

GF04529695

Iron, wire reel, 10m, diameter 0.25mm, annealed, 99.99+%

GF96915134

Iron, wire reel, 50m, diameter 0.5mm, as drawn, 99.95%

GF55306964

Iron, wire reel, 5m, diameter 0.5mm, hard, 99.99+%

GF48972987

Iron, wire reel, 20m, diameter 0.025mm, as drawn, 99.99+%

GF27207109

Iron, wire reel, 2000m, diameter 0.125mm, hard, 99.5%

GF04889751

Iron, wire reel, 0.2m, diameter 0.5mm, as drawn, 99.998%

GF51245591

Iron, wire reel, 100m, diameter 0.065mm, hard, 99.5%

GF72187612

Iron, wire reel, 0.5m, diameter 0.13mm, hard, 99.99+%

GF83584165

Iron, wire reel, 100m, diameter 0.125mm, hard, 99.5%

GF68134574

Iron, wire reel, 1000m, diameter 0.25mm, hard, 99.5%

GF60216780

Iron, foil, not light tested, 100x100mm, thickness 0.006mm, 99.85%

GF65000573

Iron, foil, not light tested, 150x150mm, thickness 0.008mm, 99.85%