MilliporeSigma
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88597

Millipore

Hydrogen peroxide solution

3%, suitable for microbiology

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Synonym(s):
Catalase Test, Catalase Test, H2O2
Empirical Formula (Hill Notation):
H2O2
CAS Number:
Molecular Weight:
34.01
Beilstein:
3587191
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Agency

according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017

Quality Level

vapor pressure

23.3 mmHg ( 30 °C)

Assay

2.8-3.2% (redox titration)

form

solution

shelf life

limited shelf life, expiry date on the label

storage condition

protect from light

concentration

1-5%

technique(s)

microbe id | specific enzyme detection: suitable

color

colorless

pH

6-8 (25 °C)

mp

0.0 °C

density

1.000 g/cm3

application(s)

clinical testing
environmental
food and beverages
veterinary

microbiology

suitability

Pneumococcus spp.
Staphylococcus spp.
bacteria

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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This Item
H100995299316989
Hydrogen peroxide solution 3%, suitable for microbiology

Millipore

88597

Hydrogen peroxide solution

vapor pressure

23.3 mmHg ( 30 °C)

vapor pressure

23.3 mmHg ( 30 °C)

vapor pressure

23.3 mmHg ( 30 °C)

vapor pressure

23.3 mmHg ( 30 °C)

assay

2.8-3.2% (redox titration)

assay

29.0-32.0% (titration)

assay

≥35% (RT)

assay

99.999% trace metals basis

form

solution

form

liquid

form

liquid

form

liquid

concentration

1-5%

concentration

30 % (w/w) in H2O

concentration

-

concentration

30-32 wt. % in water, semiconductor grade

pH

6-8 (25 °C)

pH

-

pH

-

pH

-

General description

Hydrogen peroxide is often used in microbiology for its ability to break down into water and oxygen gas. This reaction is catalyzed by the enzyme catalase, which is present in many aerobic and facultatively anaerobic bacteria. A small amount of hydrogen peroxide is added to a bacterial culture to detect the presence or absence of catalase activity in the culture.If catalase is present, it will catalyze the breakdown of the hydrogen peroxide, resulting in the formation of oxygen gas bubbles. This reaction can be visually observed as effervescence in the culture. The absence of bubbles indicates that catalase is not present in the culture.The catalase test is commonly used in microbial identification and differentiation, as different species of bacteria present varying levels of catalase activity. For example, Staphylococcus species exhibit high levels of catalase activity, while Streptococcus species do not. The catalase test is also used to differentiate between aerobic bacteria that produce oxygen as a by-product (which typically exhibit catalase activity) and facultative anaerobic bacteria that utilize oxygen but do not produce it as a by-product (which may or may not exhibit catalase activity).

Application

Hydrogen peroxide has several important microbial applications due to its strong oxidizing properties. For example:
  • Disinfectant: Hydrogen peroxide can be used as a disinfectant for surfaces, materials, and equipment in microbiology labs. When applied to surfaces, hydrogen peroxide reacts with organic materials, such as bacteria and fungi, and destroys them through oxidation.
  • Sterilization: Hydrogen peroxide is often used in sterilization procedures in microbiology, especially in the pharmaceutical and medical industries. It effectively kills spores that are resistant to many other types of disinfectants.
  • Microbial detection: Hydrogen peroxide can be used to detect catalase activity in bacteria, making it useful in microbial identification and differentiation. The species of bacteria may be distinguished based on their ability to catalyze the breakdown of hydrogen peroxide.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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