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  • Selenite-stress selected mutant strains of probiotic bacteria for Se source production.

Selenite-stress selected mutant strains of probiotic bacteria for Se source production.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) (2014-12-20)
Tünde Pusztahelyi, Szilvia Kovács, István Pócsi, József Prokisch
ABSTRACT

Selenium deficiency is a major health problem worldwide for about 1 billion people. Bacterial cells usually possess low tolerance to selenite stress and also low ability to reduce high concentrations of toxic selenite. Here, high tolerance to selenite and selenium bioaccumulation capability were developed in mutated clones of probiotic and starter bacteria including Enterococcus faecium, Bifidobacterium animalis ssp. lactis, Lactobacillus casei and Lactococcus lactis ssp. lactis by food-level strain development process and clone selection. All mutant clones possessed increased glutathione concentration and glutathione reductase activity. The selenite treatment increased further these values in L. casei mutant strain pointing at a different selenite reduction pathway and/or stress response in this organism. Considerable conversion of selenite to cell bound selenium forms with a concomitant high biomass production was detected in E. faecium and B. animalis ssp. lactis cultures. Possible application of these strains as food and feed supplements is under investigation.

MATERIALS
Product Number
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Product Description

Sigma-Aldrich
Glutathione Reductase from baker's yeast (S. cerevisiae), ammonium sulfate suspension, 100-300 units/mg protein (biuret)