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  • Monitoring of PAEMs and beta-agonists in urine for a small group of experimental subjects and PAEs and beta-agonists in drinking water consumed by the same subjects.

Monitoring of PAEMs and beta-agonists in urine for a small group of experimental subjects and PAEs and beta-agonists in drinking water consumed by the same subjects.

Journal of hazardous materials (2014-03-19)
Saou-Hsing Liou, Gordon C C Yang, Chih-Lung Wang, Yu-Han Chiu
ABSTRACT

This 5-month study contains two parts: (1) to monitor the concentrations of 11 phthalate esters metabolites (PAEMs) and two beta-agonists in human urine samples collected from a small group of consented participants including 16 females and five males; and (2) to analyze the residues of phthalate esters (PAEs) and beta-agonists in various categories of drinking water consumed by the same group of subjects. Each category of human urine and drinking water had 183 samples of its own. The analytical results showed that nine PAEMs were detected in human urine and eight PAEs were detected in drinking water samples. It was found that average concentrations of PAEMs increased as the age increased, but no significant difference between sexes. Further, using the principal component analysis, the loadings of age effect were found to be two times greater than that of gender effect in terms of four DEHP metabolites. Regarding beta-agonists of concern (i.e., ractopamine and salbutamol), they were neither detected in human urine nor drinking water samples in this study.

MATERIALS
Product Number
Brand
Product Description

Supelco
Dibutyl phthalate, PESTANAL®, analytical standard
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Benzyl butyl phthalate, analytical standard
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Bis(2-ethylhexyl) phthalate, PESTANAL®, analytical standard
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Dioctyl phthalate, ≥99.5%
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Diisononyl phthalate, ester content ≥99 % (mixture of C9 isomers), technical grade
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Benzyl butyl phthalate, 98%
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Dibutyl phthalate, 99%
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Dibutyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Dibutyl phthalate, European Pharmacopoeia (EP) Reference Standard
Plastic additive 01, European Pharmacopoeia (EP) Reference Standard
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mono-Benzyl phthalate, analytical standard
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Bis(2-ethylhexyl) phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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Bis(2-ethylhexyl) phthalate, Selectophore
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Benzyl butyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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Ammonium acetate, for molecular biology, ≥98%
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Ammonium acetate, LiChropur, eluent additive for LC-MS
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Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
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Ammonium acetate, reagent grade, ≥98%
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Ammonium acetate, 99.999% trace metals basis
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Ammonium acetate, BioUltra, for molecular biology, ≥99.0%
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Ammonium acetate, ACS reagent, ≥97%
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Ammonium acetate, ≥99.99% trace metals basis
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Ammonium acetate, BioXtra, ≥98%
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Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
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Sodium phosphate dibasic dodecahydrate, puriss. p.a., crystallized, ≥99.0% (T)
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Sodium phosphate dibasic dodecahydrate, meets analytical specification of Ph. Eur., BP, E339, 98.5-102.5% (T)
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DBP, 98% (HPLC)
Salbutamol, European Pharmacopoeia (EP) Reference Standard
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Di-n-octyl phthalate, analytical standard
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Di-n-octyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland