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09689

Sigma-Aldrich

Ammonium acetate

BioUltra, for molecular biology, ≥99.0%

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Linear Formula:
CH3CO2NH4
CAS Number:
Molecular Weight:
77.08
Beilstein/REAXYS Number:
4186741
EC Number:
MDL number:
UNSPSC Code:
12352302
eCl@ss:
39021908
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

assay

≥99.0% (calc. based on dry substance, T)
≥99.0%

form

solid

impurities

insoluble matter, passes filter test
≤0.005% KMnO4-reducing substances
≤2% water

ign. residue

≤0.01% (as SO4)

pH

6.5-7.5 (25 °C, 1 M in H2O)

mp

110-112 °C (dec.) (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5 mg/kg
nitrate (NO3-): ≤10 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤2 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.015
λ: 280 nm Amax: ≤0.010

SMILES string

N.CC(O)=O

foreign activity

DNAse, none detected
Phosphatase, none detected
Protease, none detected
RNAse, none detected

InChI

1S/C2H4O2.H3N/c1-2(3)4;/h1H3,(H,3,4);1H3

InChI key

USFZMSVCRYTOJT-UHFFFAOYSA-N

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Application


  • Modified QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry for detection of cyclopiazonic acid in feeds.: Ammonium acetate is utilized in a modified QuEChERS extraction method, improving the detection of mycotoxins in animal feed and contributing to agricultural safety and animal health (Peng et al., 2024).

  • Simultaneous determination of 31 banned veterinary drugs during egg-laying period in poultry eggs by ultra performance liquid chromatography-tandem mass spectrometry.: Highlights the role of ammonium acetate in a sensitive analytical method for monitoring drug residues in poultry eggs, crucial for food safety and consumer protection (Zhu et al., 2024).

  • Evaluation of diethyl 4-(5-bromo-1H-indol-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate: synthesis, anti-corrosion potential, and biomedical applications.: Discusses the use of ammonium acetate in the synthesis and evaluation of novel compounds with applications in materials science and medicine, showcasing its versatility in chemical synthesis (Ahamed et al., 2024).

  • Copper(II)/polyimide linked covalent organic framework as a powerful catalyst for the solvent-free microwave irradiation-based synthesis of 2,4,5-trisubstituted imidazoles.: Features the application of ammonium acetate in the development of advanced catalytic systems for green chemistry, promoting sustainable manufacturing processes (Sedaghat et al., 2023).

Biochem/physiol Actions

Ammonium acetate can be used in x-ray crystallography, optimization reagents, protein structural analysis, and proteomics. It can also be used in a study to develop a novel and simple high-performance liquid chromatography (HPLC) method for the simultaneous determination of two selective serotonin reuptake inhibitors and two serotonin-norepinephrine reuptake inhibitors in alternative samples of toxicological interest such as hair, nail clippings, and cerebrospinal fluid.

Other Notes

Elution buffer in separation of proteins by gel filtration, ammonium acetate is volatile and can be removed during lyophilization.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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C.W. Hirs
Methods in Enzymology, 47, 105-105 (1977)
Jessica Z Bereszczak et al.
Protein science : a publication of the Protein Society, 23(7), 884-896 (2014-04-10)
Hepatitis B virus core-antigen (capsid protein) and e-antigen (an immune regulator) have almost complete sequence identity, yet the dimeric proteins (termed Cp149d and Cp(-10)149d , respectively) adopt quite distinct quaternary structures. Here we use hydrogen deuterium exchange-mass spectrometry (HDX-MS) to
Elena N Kitova et al.
Journal of the American Society for Mass Spectrometry, 25(11), 1908-1916 (2014-08-20)
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of the
Xumin Zhang et al.
Analytical chemistry, 84(18), 7785-7792 (2012-08-15)
Untargeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics studies are usually carried out in both positive and negative ion modes; however, it is frequently ignored that the optimal conditions in positive ion mode and negative ion mode are often not the same.
Hong Lin et al.
Journal of the American Society for Mass Spectrometry, 23(12), 2122-2131 (2012-09-21)
Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic

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