• Ultra-small lipid nanoparticles promote the penetration of coenzyme Q10 in skin cells and counteract oxidative stress.

Ultra-small lipid nanoparticles promote the penetration of coenzyme Q10 in skin cells and counteract oxidative stress.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2014-12-17)
Silke B Lohan, Sonja Bauersachs, Sebastian Ahlberg, Nuttakorn Baisaeng, Cornelia M Keck, Rainer H Müller, Ellen Witte, Kerstin Wolk, Steffen Hackbarth, Beate Röder, Jürgen Lademann, Martina C Meinke

UV irradiation leads to the formation of reactive oxygen species (ROS). An imbalance between the antioxidant system and ROS can lead to cell damage, premature skin aging or skin cancer. To counteract these processes, antioxidants such as coenzyme Q10 (CoQ10) are contained in many cosmetics. To improve and optimize cell/tissue penetration properties of the lipophilic CoQ10, ultra-small lipid nanoparticles (usNLC) were developed. The antioxidant effectiveness of CoQ10-loaded usNLC compared to conventional nanocarriers was investigated in the human keratinocyte cell line HaCaT. Using confocal laser scanning microscopy investigations of the carriers additionally loaded with nile red showed a clear uptake into cells and their distribution within the cytoplasm. By use of the XTT cell viability test, CoQ10 concentrations of 10-50 μg/ml were shown to be non-toxic, and the antioxidant potential of 10 μg/ml CoQ10 loaded usNLC in the HaCaT cells was analyzed via electron paramagnetic resonance spectroscopy after cellular exposure to UVA (1J/cm(2)) and UVB (18 mJ/cm(2)) irradiation. In comparison with the CoQ10-loaded conventional carriers, usNLC-CoQ10 demonstrated the strongest reduction of the radical formation; reaching up to 23% compared to control cells without nanocarrier treatment. Therefore, usNLC-CoQ10 are very suitable to increase the antioxidant potential of skin.

Product Number
Product Description

Cetyl palmitate 15, European Pharmacopoeia (EP) Reference Standard
Cetyl palmitate 95, European Pharmacopoeia (EP) Reference Standard
Dioctyl ether, 99%
Palmityl palmitate, ≥99%
Cetyl Palmitate, Pharmaceutical Secondary Standard; Certified Reference Material
Span® 80, viscosity 1000-2000 mPa.s (20 °C)
Span® 80, suitable for GC
TEMPO, 98%
TEMPO, purified by sublimation, 99%