Skip to Content
MilliporeSigma
  • Spatially restricted hyaluronan production by Has2 drives epithelial tubulogenesis in vitro.

Spatially restricted hyaluronan production by Has2 drives epithelial tubulogenesis in vitro.

American journal of physiology. Cell physiology (2014-08-29)
Priscilla Soulié, Alexandra Chassot, Thomas Ernandez, Roberto Montesano, Eric Féraille
ABSTRACT

Generation of branched tubes from an epithelial bud is a fundamental process in development. We hypothesized that induction of hyaluronan synthase (Has) and production of hyaluronan (HA) drives tubulogenesis in response to morphogenetic cytokines. Treatment of J3B1A mammary cells with transforming growth factor-β1 or renal MDCK and mCCD-N21 cells with hepatocyte growth factor induced strong and specific expression of Has2. Immunostaining revealed that HA was preferentially produced at the tips of growing tubules. Inhibition of HA production, either by 4-methylumbelliferone (4-MU) or by Has2 mRNA silencing, abrogated tubule formation. HA production by J3B1A and mCCD-N21 cells was associated with sustained activation of ERK and S6 phosphorylation. However, silencing of either CD44 or RHAMM (receptor for HA-mediated motility), the major HA receptors, by RNA interference, did not alter tubulogenesis, suggesting that this process is not receptor-mediated.

MATERIALS
Product Number
Brand
Product Description

Supelco
Tretinoin, Pharmaceutical Secondary Standard; Certified Reference Material
Tretinoin, European Pharmacopoeia (EP) Reference Standard
USP
Tretinoin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Sodium selenite, SAJ first grade, ≥90.0%
Sigma-Aldrich
Sodium selenite, 99%
Sigma-Aldrich
4-Methylumbelliferone, ≥98%
Sigma-Aldrich
Sodium selenite, γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
Sigma-Aldrich
Sodium selenite, anhydrous, ≥90.0% (RT)
Sigma-Aldrich
Sodium selenite, BioReagent, suitable for cell culture, ≥98%
Hymecromone, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Has2
Sigma-Aldrich
MISSION® esiRNA, targeting human HAS2
Sigma-Aldrich
Selenous acid, 98%
Sigma-Aldrich
Selenous acid, 99.999% trace metals basis