Skip to Content
MilliporeSigma
  • In vitro effect of Paullinia cupana (guaraná) on hydrophobicity, biofilm formation, and adhesion of Candida albicans' to polystyrene, composites, and buccal epithelial cells.

In vitro effect of Paullinia cupana (guaraná) on hydrophobicity, biofilm formation, and adhesion of Candida albicans' to polystyrene, composites, and buccal epithelial cells.

Archives of oral biology (2014-12-30)
Ermelinda Matsuura, Janine Silva Ribeiro Godoy, Patrícia de Souza Bonfim-Mendonça, João Carlos Palazzo de Mello, Terezinha Inez Estivalet Svidzinski, André Gasparetto, Sandra Mara Maciel
ABSTRACT

In vitro evaluation of the effect of guaraná (GUAR) on cell surface hydrophobicity (CSH), on biofilm formation, and on adhesion of C. albicans to polystyrene, to composite resins, and to buccal epithelial cells (BEC). Lyophilised aqueous extract of GUAR was tested on C. albicans ATCC (90028). The effect of GUAR was evaluated by examining the CSH of C. albicans, as determined by microbial adhesion to hydrocarbons test, by assessing biofilm production and through adhesion assays (microplates of polystyrene, BEC and composites). One nanoparticle (Z350(®)) and two microhybrid (LLis(®), Opallis(®)) composites were tested. Scanning electron microscopy (SEM) was used to analyse adhesion of C. albicans composites. Assays were performed in triplicate and the results analysed by Chi-square test, Kruskal-Wallis test and Dunn's Multiple Comparison post hoc test at 5% significance level. GUAR did not inhibit growth of C. albicans at any concentration, but it reduced adhesion to polystyrene surface (p < 0.001). Exposure to GUAR did not change CSH and biofilm formation, but it increased adhesion of C. albicans to the nanoparticle composite (p = 0.042) and reduced its adhesion to BEC (p < 0.001). SEM confirmed an aggregatory pattern of adhesion of C. albicans to composites. GUAR increased the adhesion of C. albicans to the surface of the nanoparticle composite. However, it reduced the adhesion of C. albicans to BEC and to polystyrene, which reveals its potential use in prevention of oral diseases.

MATERIALS
Product Number
Brand
Product Description

Supelco
D-(+)-Glucose, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
Pricing and availability is not currently available.
Sigma-Aldrich
Hexadecane, ReagentPlus®, 99%
Pricing and availability is not currently available.
USP
Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
Supelco
Hexadecane, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, tested according to Ph. Eur.
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, ACS reagent
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, Hybri-Max, powder, BioReagent, suitable for hybridoma
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC), BioXtra
Pricing and availability is not currently available.
Sigma-Aldrich
D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, JIS first grade, 94.8-95.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, JIS special grade, 94.8-95.8%
Pricing and availability is not currently available.
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Pricing and availability is not currently available.
Sigma-Aldrich
Hexadecane, anhydrous, ≥99%
Pricing and availability is not currently available.
Supelco
Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Pricing and availability is not currently available.
USP
Dextrose, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
Dextrose, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
Pricing and availability is not currently available.
Supelco
Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ACS reagent, prima fine spirit, without additive, F15 o1
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for absorption spectrum analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, SAJ super special grade
Pricing and availability is not currently available.