Skip to Content
MilliporeSigma

Interactions of arene ruthenium metallaprisms with human proteins.

Organic & biomolecular chemistry (2014-11-21)
Lydia E H Paul, Bruno Therrien, Julien Furrer
ABSTRACT

Interactions between three hexacationic arene ruthenium metallaprisms, [(p-cymene)6Ru6(tpt)2(dhnq)3](6+), [(p-cymene)6Ru6(tpt)2(dhbq)3](6+) and [(p-cymene)6Ru6(tpt)2(oxa)3](6+), and a series of human proteins including human serum albumin, transferrin, cytochrome c, myoglobin and ubiquitin have been studied using NMR spectroscopy, mass spectrometry and circular dichroism spectroscopy. All data suggest that no covalent adducts are formed between the proteins and the metallaprisms. Indeed, in most cases electrostatic interactions, leading to precipitation of protein-metallaprism aggregates, have been observed. In addition, with the smallest proteins, ubiquitin, myoglobin and cytochrome c, the presence of the hexacationic arene ruthenium metallaprisms induces structural changes of the proteins, as emphasized by circular dichroism. The results suggest that proteins are certainly a biological target for these metalla-assemblies.

MATERIALS
Product Number
Brand
Product Description

Supelco
Water, for HPCE, for luminescence, suitable for UV/Vis spectroscopy
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, tested according to Ph. Eur.
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, for cell biology, sterile ultrafiltered
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, PCR Reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, Nuclease-Free Water, for Molecular Biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, sterile-filtered, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sinapic acid, ≥98%, powder
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sinapic acid, matrix substance for MALDI-MS, ≥99.0% (T)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Density Standard 998 kg/m3, H&D Fitzgerald Ltd. Quality
SKU
Pack Size
Availability
Price
Quantity
Supelco
Water, for TOC analysis
SKU
Pack Size
Availability
Price
Quantity
Supelco
Water, suitable for ion chromatography
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, for molecular biology, sterile filtered
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
E-Toxate Water, endotoxin, free
SKU
Pack Size
Availability
Price
Quantity
Supelco
Water, ACS reagent, for ultratrace analysis
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sinapic acid, matrix substance for MALDI-MS, ≥99.5%, Ultra pure
SKU
Pack Size
Availability
Price
Quantity
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
SKU
Pack Size
Availability
Price
Quantity
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, BioPerformance Certified
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, ACS reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, Deionized
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Water, HPLC Plus
SKU
Pack Size
Availability
Price
Quantity
Supelco
trans-Sinapic acid, analytical standard
SKU
Pack Size
Availability
Price
Quantity