- Formulation of highly functionalizable DNA nanoparticles based on 1,2-dithiolane derivatives.
Formulation of highly functionalizable DNA nanoparticles based on 1,2-dithiolane derivatives.
Chembiochem : a European journal of chemical biology (2015-02-18)
Coralie Charrat, Anaïs Biscotti, Guilhem Godeau, Jacques Greiner, Pierre Vierling, Jean-Marie Guigonis, Christophe Di Giorgio
PMID25689838
ABSTRACT
We describe the formulation of synthetic virus models based on ionic compounds bearing the polymerizable 1,2-dithiolane moiety. First, cationic amphiphiles containing the polymeric inducer were prepared and used to efficiently condense a DNA plasmid (pDNA) into a highly monodisperse population of small polymeric cationic DNA nanoparticles (NPs; Dh ∼100 nm). These nonspecific cationic particles were then functionalized with anionic PEGylated conjugates, also based on the 1,2-dithiolane motifs, in order to produce stable and fully dispersible stealth DNA nanoparticles. Our results show that both ionic interactions and polymerization based on the 1,2-dithiolane pattern occur and that they produce highly functionalizable nonviral DNA NPs.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Sulfuric acid, puriss. p.a., for determination of Hg, ACS reagent, reag. ISO, reag. Ph. Eur., 95.0-97.0%
Sigma-Aldrich
N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Sigma-Aldrich
Ethanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)