Herbal medicine Guan Chang Fu Fang enhances 5-fluorouracil cytotoxicity and affects drug-associated genes in human colorectal carcinoma cells.

Herbal medicine Guan Chang Fu Fang enhances 5-fluorouracil cytotoxicity and affects drug-associated genes in human colorectal carcinoma cells.

Oncology letters (2015-01-27)

Chen Yu, Shen-Lin Liu, Ming-Hao Qi, Xi Zou, Jian Wu, Jing Zhang

PMID25621039

ABSTRACT

Guan Chang Fu Fang (GCFF) is a natural compound, which is extracted from three medicinal plants,

MATERIALS

Product Number

Brand

Product Description

W200603

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

270474

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, 99.93%

W200611

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

82762

Supelco

Methanol, analytical standard

48075

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

F6627

Sigma-Aldrich

5-Fluorouracil, ≥99% (HPLC), powder

G5251

Sigma-Aldrich

DL-Glyceraldehyde 3-phosphate solution, 45-55 mg/mL in H₂O

F8423

Sigma-Aldrich

Fluorouracil, meets USP testing specifications

2858

Sigma-Aldrich

Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

09-0790

Sigma-Aldrich

Ethanol, JIS special grade, 94.8-95.8%

01-0280

Sigma-Aldrich

Acetic acid, JIS special grade, ≥99.7%

09-0800

Sigma-Aldrich

Ethanol, JIS first grade, 94.8-95.8%

01-0300

Sigma-Aldrich

Acetic acid, ≥99.7%, suitable for amino acid analysis

45726

Sigma-Aldrich

Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)

01-0290

Sigma-Aldrich

Acetic acid, SAJ first grade, ≥99.0%

01-0330

Sigma-Aldrich

Acetic acid, 99.5-100.0%

01-0310

Sigma-Aldrich

Acetic acid, ≥99.7%

01-0320

Sigma-Aldrich

Acetic acid, ≥99.7%

01-0350

Sigma-Aldrich

Acetic acid solution, 1 M, 1 N

19-2410

Sigma-Aldrich

Methanol, JIS special grade, ≥99.8%

19-2420

Sigma-Aldrich

Methanol, SAJ first grade, ≥99.5%

19-2450

Sigma-Aldrich

Methanol, JIS 300, ≥99.8%, for residue analysis

606766

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

19-2470

Sigma-Aldrich

Methanol, suitable for HPLC

19-2400

Sigma-Aldrich

Methanol, SAJ special grade

PHR1070

Supelco

Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material

717975

Sigma-Aldrich

Methanol, NMR reference standard

1012772

USP

Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard

650609

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles