Skip to Content
MilliporeSigma
  • Engineered electrical conduction tract restores conduction in complete heart block: from in vitro to in vivo proof of concept.

Engineered electrical conduction tract restores conduction in complete heart block: from in vitro to in vivo proof of concept.

Journal of the American College of Cardiology (2014-12-20)
Eugenio Cingolani, Vittoria Ionta, Ke Cheng, Alessandro Giacomello, Hee Cheol Cho, Eduardo Marbán
ABSTRACT

Cardiac electrical conduction delays and blocks cause rhythm disturbances such as complete heart block, which can be fatal. Standard of care relies on electronic devices to artificially restore synchrony. We sought to create a new modality for treating these disorders by engineering electrical conduction tracts designed to propagate electrical impulses. This study sought to create a new approach for treating cardiac conduction disorders by using engineered electrical conduction tracts (EECTs). Paramagnetic beads were conjugated with an antibody to gamma-sarcoglycan, a cardiomyocyte cell surface antigen, and mixed with freshly isolated neonatal rat ventricular cardiomyocytes. A magnetic field was used to pattern a linear EECT. In an in vitro model of conduction block, the EECT was patterned so that it connected 2 independently beating neonatal rat ventricular cardiomyocyte monolayers; it achieved coordinated electrical activity, with action potentials propagating from 1 region to the other via EECT. Spiking the EECT with heart-derived stromal cells yielded stable structures with highly reproducible conduction velocities. Transplantation of EECTs in vivo restored atrioventricular conduction in a rat model of complete heart block. An EECT can re-establish electrical conduction in the heart. This novel approach could, in principle, be used not only to treat cardiac arrhythmias but also to repair other organs.

MATERIALS
Product Number
Brand
Product Description

USP
Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, JIS first grade, 94.8-95.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, JIS special grade, 94.8-95.8%
Pricing and availability is not currently available.
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Pricing and availability is not currently available.
Supelco
Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Pricing and availability is not currently available.
Sigma-Aldrich
Hexamethyldisilazane, produced by Wacker Chemie AG, Burghausen, Germany, ≥97.0% (GC)
Pricing and availability is not currently available.
Supelco
Hexamethyldisilazane, for GC derivatization, LiChropur, ≥99.0% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ACS reagent, prima fine spirit, without additive, F15 o1
Pricing and availability is not currently available.
Sigma-Aldrich
Hexamethyldisilazane, reagent grade, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Hexamethyldisilazane, ReagentPlus®, 99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for absorption spectrum analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, SAJ super special grade
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, JIS 300, ≥99.5%, for residue analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, JIS 1000, ≥99.5%, for residue analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for fluorescence
Pricing and availability is not currently available.
Supelco
Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, purum, absolute ethanol, denaturated with 2% 2-butanone, A15 MEK1, ≥99.8% (based on denaturant-free substance)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, puriss. p.a., absolute, ≥99.8% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, HPLC/spectrophotometric grade
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Pricing and availability is not currently available.
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Pricing and availability is not currently available.