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  • Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.

Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.

Molecules and cells (2015-10-09)
Jing Guo, Hongjuan He, Qi Liu, Fengwei Zhang, Jie Lv, Tiebo Zeng, Ning Gu, Qiong Wu
ABSTRACT

Most imprinted genes are concerned with embryonic development, especially placental development. Here, we identified a placenta-specific imprinted gene Qpct. Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5b-E11.5. Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5. Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta. ChIP assay results suggest that the modification of histone H3K4me3 can result in maternal activating of Qpct.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
3,4,4′-Trichlorocarbanilide, 99%
Sigma-Aldrich
Anti-QPCT antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution