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  • Identification of a histone acetyltransferase as a novel regulator of Drosophila intestinal stem cells.

Identification of a histone acetyltransferase as a novel regulator of Drosophila intestinal stem cells.

FEBS letters (2013-03-29)
Yansen Ma, Zhi Chen, Yaping Jin, Wei Liu
ABSTRACT

One of the major challenges in stem cell research is to decipher the controlling mechanisms/genes of stem cell homeostasis. Through an RNAi mediated genetic screen of living animals, we identified an evolutionarily conserved histone acetyltransferase Atac2 as a novel regulator of Drosophila intestinal stem cells (ISCs). Expression of Atac2-RNAi or a dominant negative allele of Atac2 generated more ISCs, while excessive Atac2 or a histone deacetylase inhibitor promoted ISC differentiation without affecting ISC survival or lineage specification. These findings extend our knowledge of epigenetic mechanisms in stem cell regulation.

MATERIALS
Product Number
Brand
Product Description

Roche
DIG RNA Labeling Kit (SP6/T7), sufficient for 2 x 10 labeling reactions, kit of 1 (12 components), suitable for hybridization, suitable for Southern blotting
Roche
Anti-Digoxigenin-Rhodamine, Fab fragments, from sheep