11207750910
Roche
Anti-Digoxigenin-Rhodamine, Fab fragments
from sheep
Synonym(s):
anti-digoxigenin, digoxigenin
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
sheep
Quality Level
conjugate
rhodamine conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized (clear, red solution after reconstitution)
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
General description
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.
Specificity
Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).
Application
Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
- Digoxigenin-labeled sugars in glycoconjugate research
- Fluorescent in situ hybridization (FISH)
- Immunohistocytochemistry
- In situ hybridization
- DNA fiber assay
Preparation Note
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
- Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
- Immunohistocytochemistry: 20 to 50 μg/ml
- In situ hybridization: 20 to 50 μg/ml
Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
Reconstitution
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
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Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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