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D8156

Sigma-Aldrich

Monoclonal Anti-Digoxin antibody produced in mouse

clone DI-22, ascites fluid

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Synonym(s):
Monoclonal Anti-Digoxin

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DI-22, monoclonal

contains

15 mM sodium azide

technique(s)

dot blot: suitable
flow cytometry: suitable
indirect ELISA: 1:10,000 using digoxin-BSA
indirect ELISA: 1:2,500 using digoxigenin-transferrin

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • dot blot
  • flow cytometry
  • fluorescence in situ hybridization (FISH)
  • DNA hybridization
  • in-situ hybridization (ISH)
  • immunodetection of the probe and chromosome X

Biochem/physiol Actions

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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